Journal: Immunity

Article Title: An interleukin-9-ZBTB18 axis promotes germinal center development of memory B cells.

doi: 10.1016/j.immuni.2025.02.021

Figure Lengend Snippet: Figure 3. ZBTB18 does not impinge on B cell activation or GC formation (A) Intracellular calcium responses of naive B cells from Cd79acre/+ and Cd79acre/+Zbtb18fl/flmice upon anti-IgM stimulation. Cells were pooled from 2 to 3 mice. Data represent three independent experiments. (B and C) Proliferation of Cd79acre/+ and Cd79acre/+Zbtb18fl/flnaive B cells 72 h after anti-IgM, LPS, or anti-CD40 stimulation. Shown are representative CellTrace Violet dilution profiles (B) and summary data of the fraction of divided cells (C). Each symbol represents one independent experiment with cells pooled from 2 to 3 mice per genotype. (D–H) The primary response of Aicda+/CreERT2;Zbtb18fl/fl;Rosa26-Ai14 and Aicda+/CreERT2;Zbtb18+/+;Rosa26-Ai14 mice. (D) The experimental design. Tamoxifen was given every other day starting from day 3. (E) Representative FACS profiles for gating GCs, GCMP cells, and NP-specific tdTomato+ MBCs. Fractional abundance of GCs in total B220+ cells (F), GCMP cells in GCs (G), and total MBCs or tdTomato+ MBCs in total B220+ cells (H). Data are pooled from 3 independent experiments.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: S1pr2-creERT2 Dr. T. Kurosaki; Osaka University N/A Mouse: B1-8hi Dr. M. Nussenzweig, Rockefeller University N/A Mouse: Cd79acre/+ Dr. M. Reth, University of Freiburg N/A Mouse: Il9-/- This paper N/A Mouse: Zbtb18fl/fl This paper N/A Mouse: Zbtb18Flag-in/Flag-in This paper N/A Oligonucleotides qPCR primers, see Table S1 This paper N/A ChIP-qPCR primers, see Table S1 This paper N/A Software and algorithms Adobe Illustrator Adobe https://www.adobe.com/products/illustrator.html Prism v8 Graphpad http://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 Tree Star https://www.flowjo.com/ Imaris Bitplane AG http://www.bitplane.com/imaris/imaris RRID:SCR_007370 Image J Wayne Rasband https://imagej.net/software/fiji/downloads Other BD FACS Aria III Cell Sorter BD Biosciences N/A LSR II cytometer BD Biosciences N/A iMark plate reader Bio-Rad Cat# 1681135 Aurora cytometer Cytek Biosciences N/A 96-well ELISA plates Costar Cat# 42592 CellDrop BF Brightfield Cell Counter DeNovix N/A Leica STELLARIS 8 Falcon confocal microscope Leica N/A Qubit 4 Fluorometer Invitrogen N/A Immunity 58, 1–14.e1–e6, April 8, 2025 e3 were immunized intraperitoneally with 10 mg NP-OVA (Biosearch Technologies) in PBS.

Techniques: Activation Assay

Journal: Immunity

Article Title: An interleukin-9-ZBTB18 axis promotes germinal center development of memory B cells.

doi: 10.1016/j.immuni.2025.02.021

Figure Lengend Snippet: Figure 5. ZBTB18 promotes survival of GCMP cells (A) The relative mRNA expression of Casp3 and Bid by qRT-PCR, with wild type set as 1. Each dot represents an independent sort of 200 cells. (B) Diagrams of 2-kb promoters of indicated gene, with ZBTB18-binding motifs highlighted and primers for ChIP-qPCR indicated by arrows. (C) Normalized luciferase activities, transcriptionally driven by 2-kb promoters with or without exogenous ZBTB18 transduced in 293T cells. Data are from 3 independent experiments, p values by t tests. (D) ChIP-qPCR analysis of ZBTB18-regulated apoptosis-promoting genes in MBCs from Zbtb18FLAG-in/FLAG-in mice. Shown are enrichment of promoter frag- ments, with immunoprecipitation performed using the anti-FLAG or control IgG antibody. One of two independent experiments with similar results is shown. (E) Representative FACS profiles (left) and summary data (right) of GCMP cells expressing active caspases. In scatter plots, each dot represents one mouse. Data are pooled from four independent experiments. p values by t tests, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: S1pr2-creERT2 Dr. T. Kurosaki; Osaka University N/A Mouse: B1-8hi Dr. M. Nussenzweig, Rockefeller University N/A Mouse: Cd79acre/+ Dr. M. Reth, University of Freiburg N/A Mouse: Il9-/- This paper N/A Mouse: Zbtb18fl/fl This paper N/A Mouse: Zbtb18Flag-in/Flag-in This paper N/A Oligonucleotides qPCR primers, see Table S1 This paper N/A ChIP-qPCR primers, see Table S1 This paper N/A Software and algorithms Adobe Illustrator Adobe https://www.adobe.com/products/illustrator.html Prism v8 Graphpad http://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 Tree Star https://www.flowjo.com/ Imaris Bitplane AG http://www.bitplane.com/imaris/imaris RRID:SCR_007370 Image J Wayne Rasband https://imagej.net/software/fiji/downloads Other BD FACS Aria III Cell Sorter BD Biosciences N/A LSR II cytometer BD Biosciences N/A iMark plate reader Bio-Rad Cat# 1681135 Aurora cytometer Cytek Biosciences N/A 96-well ELISA plates Costar Cat# 42592 CellDrop BF Brightfield Cell Counter DeNovix N/A Leica STELLARIS 8 Falcon confocal microscope Leica N/A Qubit 4 Fluorometer Invitrogen N/A Immunity 58, 1–14.e1–e6, April 8, 2025 e3 were immunized intraperitoneally with 10 mg NP-OVA (Biosearch Technologies) in PBS.

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, ChIP-qPCR, Luciferase, Immunoprecipitation, Control

Journal: Data in Brief

Article Title: Characterization data on the topical carrier DDC642

doi: 10.1016/j.dib.2016.03.091

Figure Lengend Snippet:

Article Snippet: How data were acquired , Gel imaging system (ChemiDoc-It Imager, UVP or ChemiDoc XRS+, Bio-Rad), Cryo-electron microscopy (FEI Tecnai 20, FEI or CM120, Philips), Microplate reader (FLUOstar OPTIMA, BMG Labtech or EnVision Multilabel Plate Reader, Perkin Elmer), Flow cytometer (BD LSRFortessa, BD Biosciences), Real-time PCR detection system (MyiQ iCycler, Bio-Rad), Dynamic light scattering system (Zetasizer Nano series, Malvern)..

Techniques: Liposomes, Imaging, Cryo-Electron Microscopy, Flow Cytometry, Real-time Polymerase Chain Reaction, Encapsulation, Cell Culture, In Vitro, Transfection, Nucleic Acid Electrophoresis, Western Blot, Diffusion-based Assay